刊行物

刊行物
(Lignin)
Vol.3(2022)
Original article
4-Hydroxybenzoic Acid Secreted from Trametes versicolor Catalyzes the Oxidation of a Nonphenolic β-O-4 Lignin Model Dimer as Laccase Mediator

Shingo Kawai, Megumi Miyazaki, Yoshimi Hayashi, Miyuki Matsunaga, Haruka Hirashita, Tomoaki Nishida, Yuko Yoneda
Pages: 1-8
Published online: 17 May 2022

Abstract: To detect natural laccase mediator, the extracellular culture fluid of Trametes versicolor laccase production medium was fractionated using degradation of anthracene which can not be degraded with laccase alone. As a result, 4-hydroxybenzoic acid (HBA) was isolated from the culture fluid, and it was clear that HBA acted as a natural mediator and the degradation efficiency of anthracene by laccase greatly increased. The laccase-HBA system could also oxidized the nonphenolic β-O-4 lignin model dimer to pruduce the Cα-oxidation, β-ether cleavage and aromatic ring cleavage products. The oxidation mechanisms of the laccase-HBA system are very similar to those for lignin peroxidase and laccase-1-hydroxybenzotriazol (HBT) system.
PDF